OPENS A NEW DIMENSION
THE AGE OF HANGING DROP TOWERS IS OVER
Stable gene expression needs regular clusters in 3D.
Our technology gives you thousands of these with ease!
Stem Cell Quality Clustering
Medium Change Possibility
The fusion of geometry with biology - every cell finds its niche
Physiological environment thanks to rounded bottoms
No adhesion thanks to high-end ultra low attachment nanocoating
HOURS WILL BECOME MINUTES
One single pipet move gives you 750 cell clusters. This is almost as much as you see here on the left. Even the fastest multichannel repeater is 60x slower.
Designed and made in Switzerland
Our geometry allows the whole well bottom to be covered with microwells. No cell is lost on dead surfaces. Your cells travel with pure gravity into a physiological environment created with rounded bottoms. The essence of years of experience and dedication is in your hands now.
Background picture: electron microscopy of original stamping tool.
THE PERFECT 3D ENVIRONMET FOR YOUR CELLS
FIELDS OF USE
UNCOVER THE POTENTIAL
3D Stem cell differentiation and maintenance
Any somatic 3D cell type application like cancer research, drug and toxicity screening
All kinds of analyses where a multitude of regular clusters are needed like histology, protein analysis, mutation analysis, clonal expansion and many more
Soon cGMP compliant: somatic and stem cell cluster transplantation
compared to hanging drops technology
MEDIUM CHANGE IS POSSIBLE
fast and could be automated
versus a traditional 8 drop multichannel repeater
perfect clustering and no adhesion (various stem and somatic cells tested)
MONEY SAVINGS UP TO
Material and labour cost reduction
compared to most other technologies and products
UP TO 400 TIMES
From the top of the homepage until here: all of these clusters are in one Sphericalplate 5D!
We believe that regenerative medicine is one of the biggest promises for future medical therapies. Everyone should get access to it and we want to contribute to this goal with all our passion and skills.
Stem cells are the source of our physical life and harbour unlimited potential. But if they do not receive a lot of care within a totally physiological environment they may seriously harm. We believe that we can avoid this by meticulously studying all the factors influencing stem cell differentiation and changing things to be changed.
VISION BECOMES REALITY
A COSY HOME FOR CELLS
Cell friendly ultra low attachment surface
US Pat. 8,911,690 B2
12 x 750 microwells
= 9000 microwells
Cell inspired design
Cell friendly rounded bottoms
ISO 11192 compliant
Designed and made
Extreme ease of use
We are a strong team with one vision! If you would like to join our team, get in contact with us.
PATRICK KUGELMEIER, DR. MED.
Director of Science
Researcher and Entrepreneur
Transplant and stem cell research
Founder Kugelmeiers AG
SANDRA H. KUGELMEIER-HELLER, CETC
Director of Health
Transplant Specialist and Entrepreneur
Co-Founder Donor Care Association
Founder Kugelmeiers AG
MARTIN A. MEIER, MAS ETH, MBA HSG
Director of Technology
Entrepreneur & Architect
Founder Raumgleiter AG
Founder Kugelmeiers AG
Board Member of Greenpeace Switzerland
DR. RICHARD ZÜLLIG, PHD
Head of Laboratory
Clinical Transplantation & Resarch University Hospital Zürich
Scientific & Clinical Support
Establishment of modified clinical islet transplant case
Strong Scientific Expertise
Our journey began with the young medical student Patrick Kugelmeier doing his thesis in islet transplantation. The joy of research led to an MD-PhD program for the early differentiation of stem cells. Cell culture technologies at that time didn't allow for good data reproducibility. Consequent cell culture analysis itself finally led to the insight that we fundamentally need to rethink how we treat and culture our cells. We are at the tipping point of a new medical era; stem cell therapy in humans. This demands highest ethics, because these cells become parts of our bodies.
So proper cell differentiation and therefore avoidance of tumor formation is of utmost importance. BUT: genetic alterations are already happening on the level of poorly conceived cell culture methods, so we need to start here.
It took years of sweat and tears to develop a first step into scalable cluster formation in stem cell quality, which is now the Sphericalplate 5D. But it was worth it.
From our experience in human clinical islet cell transplantation for the treatment of Diabetes we found that large islet size promotes hypoxic damage and subsequent transplant loss. We therefore developed a plate to reaggregate islets of defined sizes in thousands of microwells. (Lehmann et al. 2007)
From our experience with differentiation studies of stem cells, we found that cell culture form, cluster size and cell culture surface are decisive for communication in between stem cells and their differentiation. We therefore insisted in developing microwells with rounded bottoms creating a special, patented geometry. An additional nanocoating allows perfect cell aggregation. (Paper in Review)
The vision to create rounded bottoms of the microwells was inspi-rational with at that time no scientific evidence.
After development of plate prototypes, we tried to prove that vision that rounded bottoms are needed by using differentiating stem cells and real time PCR – but with the targets at hand at that time we could not provide that evidence.
But the scientific community was not sleeping – and the group from Kara McCloskey provided the evidence that our way was the right one (Silin Sa 2012): Stem cell fate is influenced of the culture vessel form!
The scientific evidence that stem cells communicate in a spatial manner is growing exponentially. If we ever want to use stem cells therapeutically in a safe way, we need to study them cluster size controlled in 3D. The literature in PubMed for "Stem cells 3D" speaks for itself.